Exacto Test HIV is a self-testing rapid test for HIV that can be done in less than 10 min with micro-sampling of 5 µL of whole blood with an easy-to-use inverted cup collector. Sample collection does not require any training and is no more complicated than oral fluid collection while generating a higher performance level. In addition, the cassette format with procedure information printed on the plastic makes acceptance of this test higher among general population. Field studies in Africa have confirmed high level of acceptance and local African language versions of instructions for use have been developed. In addition, access to a YouTube demo is presented in the instructions. Exacto Test HIV is a third generation of HIV rapid test technology. Thanks to a double sandwich detection system, Exacto Test HIV allows very early detection in primary infected patients.